Wednesday, October 30, 2013

Follow-Up on Hershey and Chase

I wanted to post a link to a cool scientific paper that I saw when I was doing research for the previous post on Martha Chase.  It is called "A Single-Molecule Hershey-Chase Experiment" by Van Valen, et al., and was published in the journal Current Biology.  It is available to read here for free. 

These researchers took phages with DNA that was labeled with a fluorescent cyanine dye.  Using a fluorescence microscope, they monitored the time it takes for the bacteriophages to inject the DNA into E. coli bacteria cells (approximately 5-10 seconds).  Figure 1 of the paper shows their experimental scheme while Figure 2 shows an actual experiment.  It is definitely worth checking out if you are interested in bacteriophages or the Hershey-Chase experiment.    


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